Coding

Part:BBa_K5255000:Design

Designed by: Ruiqi Cao   Group: iGEM24_XJTLU-CHINA   (2024-09-19)


Lacs1-his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 974
    Illegal XbaI site found at 304
    Illegal PstI site found at 1799
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 974
    Illegal PstI site found at 1799
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 974
    Illegal BglII site found at 1505
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 974
    Illegal XbaI site found at 304
    Illegal PstI site found at 1799
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 974
    Illegal XbaI site found at 304
    Illegal PstI site found at 1799
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the need for heterologous expression of the protein, codon optimization is required to make its presence and expression more stable in yeast. Optimization (for Saccharomyces cerevisiae(Yeast)) and routine synthesis of LACS1 gene, adding 5' (BamHI) and 3'UTR ([TG]) and 3' (NotI), the gene was cloned into vector pYES2CT (Ampicillin) by 5' BamHI and 3' NotI, and the mini-scale recombinant plasmid was prepared DNA and puncture bacteria containing the recombinant plasmid.


Source

https://www.ncbi.nlm.nih.gov/nuccore/NM_130292.4

References